Studies conducted on bench installations in flowing round-the-clock mode have shown the possibility, expediency and reliability of biological treatment application in methanol removal using free-floating activated sludge and immobilized microorganisms at its ultimate initial concentration of 800 mg/l. According to the data obtained by using 2-stage biological oxidation system for the total residence time of 43.6 h residual methanol values of about 0.1 mg/l were obtained, which corresponds to the MPC of methanol for discharge into water bodies of commercial fishing significance. Considering the fact that biological treatment is a traditional and cost-effective technology it is recommended to use it for removal of high concentrations of methanol from industrial wastewaters.